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Upregulation of MHC class II antigen in human melanoma xenografts treated systemically with anle138b, and expression of α-synuclein, MHC class II, and MHC class I proteins in melanoma <t>TMA</t> cores. (a) A tissue section from a nontreated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (b) A tissue section from an anle138b-treated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (c) Fluorescence intensity level of expression of proteins – α-synuclein (pseudocolored red), MHC II (pseudocolored yellow), MHC I (pseudocolored green) – in areas, encircled by a white line, in tissue sections from three of the Stage II and from one of the Stage IV melanoma TMA cores in which α-synuclein or vice versa, MHC II, or MHC I protein was expressed more strongly. TMA, tissue <t>microarray.</t>
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Upregulation of MHC class II antigen in human melanoma xenografts treated systemically with anle138b, and expression of α-synuclein, MHC class II, and MHC class I proteins in melanoma <t>TMA</t> cores. (a) A tissue section from a nontreated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (b) A tissue section from an anle138b-treated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (c) Fluorescence intensity level of expression of proteins – α-synuclein (pseudocolored red), MHC II (pseudocolored yellow), MHC I (pseudocolored green) – in areas, encircled by a white line, in tissue sections from three of the Stage II and from one of the Stage IV melanoma TMA cores in which α-synuclein or vice versa, MHC II, or MHC I protein was expressed more strongly. TMA, tissue <t>microarray.</t>
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Upregulation of MHC class II antigen in human melanoma xenografts treated systemically with anle138b, and expression of α-synuclein, MHC class II, and MHC class I proteins in melanoma <t>TMA</t> cores. (a) A tissue section from a nontreated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (b) A tissue section from an anle138b-treated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (c) Fluorescence intensity level of expression of proteins – α-synuclein (pseudocolored red), MHC II (pseudocolored yellow), MHC I (pseudocolored green) – in areas, encircled by a white line, in tissue sections from three of the Stage II and from one of the Stage IV melanoma TMA cores in which α-synuclein or vice versa, MHC II, or MHC I protein was expressed more strongly. TMA, tissue <t>microarray.</t>
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Upregulation of MHC class II antigen in human melanoma xenografts treated systemically with anle138b, and expression of α-synuclein, MHC class II, and MHC class I proteins in melanoma TMA cores. (a) A tissue section from a nontreated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (b) A tissue section from an anle138b-treated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (c) Fluorescence intensity level of expression of proteins – α-synuclein (pseudocolored red), MHC II (pseudocolored yellow), MHC I (pseudocolored green) – in areas, encircled by a white line, in tissue sections from three of the Stage II and from one of the Stage IV melanoma TMA cores in which α-synuclein or vice versa, MHC II, or MHC I protein was expressed more strongly. TMA, tissue microarray.

Journal: Melanoma Research

Article Title: Interfering with aggregated α-synuclein in advanced melanoma leads to a major upregulation of MHC class II proteins

doi: 10.1097/CMR.0000000000000982

Figure Lengend Snippet: Upregulation of MHC class II antigen in human melanoma xenografts treated systemically with anle138b, and expression of α-synuclein, MHC class II, and MHC class I proteins in melanoma TMA cores. (a) A tissue section from a nontreated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (b) A tissue section from an anle138b-treated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (c) Fluorescence intensity level of expression of proteins – α-synuclein (pseudocolored red), MHC II (pseudocolored yellow), MHC I (pseudocolored green) – in areas, encircled by a white line, in tissue sections from three of the Stage II and from one of the Stage IV melanoma TMA cores in which α-synuclein or vice versa, MHC II, or MHC I protein was expressed more strongly. TMA, tissue microarray.

Article Snippet: Four melanoma tissue microarray (TMA) slides ( https://www.biomax.us/tissue-arrays/Melanoma/ME811 ), each containing two tissue sections from the same 39 cases of melanoma, and tissue sections from three cases of normal skin tissue were heated at 60 °C, deparaffinized and rehydrated.

Techniques: Expressing, Fluorescence, Microarray